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Sperm acrosomal marker enzyme

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Several approaches were next used to confirm the presence of amyloid in AM isolated from cauda epididymal spermatozoa. This is usually done after staining with a fluoresceinated lectin such as FITC-PNA, FITC-PSA, FITC-ConA, or fluoresceinated antibody such as FITC-CD Sperm was subjected to two in vitro fertilization IVF conditions; capacitation and control condition as previously described [20]. Physiol Bethesda ; 25 3: Copyright Tsai et al. J Biol Chem ;

During the acrosome reaction the contents of the acrosome are released outwardly. Review Article First Online: Birefringence microscopy, [7] flow cytometry [8] or fluorescence microscopy can be used for assessing the shedding of the acrosome or "acrosome reaction" of a sperm sample. Baba T, Azuma S, Kashiwabara S, Toyoda Y Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. Mol Hum Reprod FSH from the pituitary gland.

Acrosome markers of human sperm

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Study sets matching "the acrosome of a sperm cell contains"

    Gao Z, Garbers DL Species diversity in the structure of zonadhesin, a sperm-specific membrane protein containing multiple cell adhesion molecule-like domains. It undergoes its secondary meiotic division, and the two haploid nuclei paternal and maternal fuse to form a zygote. The acrosome reaction can be stimulated in vitro by substances a sperm cell may encounter naturally such as progesterone or follicular fluid , as well as the more commonly used calcium ionophore A Baba D, Kashiwabara S, Honda A et al Mouse sperm lacking cell surface hyaluronidase PH can pass through the layer of cumulus cells and fertilize the egg. Linder B, Bammer S, Heinlein UA Delayed translation and posttranslational processing of cyritestin, an integral transmembrane protein of the mouse acrosome. Mori E, Baba T, Iwamatsu A, Mori T Purification and characterization of a kDa protein, sp38, with zona pellucida-binding property from porcine epididymal sperm. With the exception of acid phosphatase, no alteration in enzyme activity occurred after 4 h of incubating the spermatozoa under capacitation conditions although gamete fusion took place. Toshimori K Dynamics of the mammalian sperm membrane modification leading to fertilization: Foster JA, Friday BB, Maulit MT et al AM67, a secretory component of the guinea pig sperm acrosomal matrix, is related to mouse sperm protein sp56 and the complement component 4-binding proteins.

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